2020/12/21 12:03:21
提取動物關(guān)節(jié)軟骨組織總RNA
Source：Shanghai Jingxin
提取動物關(guān)節(jié)軟骨組織總RNA
實驗?zāi)康?/strong>
研磨破碎軟骨組織（大鼠膝關(guān)節(jié)軟骨），提取其總RNA。
實驗原理
充分磨碎軟骨樣品（大鼠膝關(guān)節(jié)軟骨），再用相關(guān)試劑提取其總RNA。
實驗材料和器具
樣品：大鼠膝關(guān)節(jié)軟骨
儀器：多樣品組織研磨儀（上海凈信，Tissuelyser-24）
耗材：研磨罐，研磨珠
試劑：液氮若干
實驗步驟
需了解具體步驟，請致電廠家
實驗結(jié)果
采用本實驗方法提取的動物關(guān)節(jié)軟骨組織總RNA的收率高，并且純度高、質(zhì)量、完整性好，可以滿足分子生物學(xué)研究應(yīng)用。
總RNA甲醛變性膠電泳結(jié)果
Ⅱ型膠原PCR擴增產(chǎn)物電泳結(jié)果
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