實(shí)驗(yàn)?zāi)康模?/strong>研磨肌肉組織,更有效快速的提取核酸蛋白。
實(shí)驗(yàn)地點(diǎn):清華大學(xué)
實(shí)驗(yàn)材料和器具
樣品:小鼠肌肉組織
儀器:高通量組織研磨儀(上海凈信,TISSUELYSER-24)
耗材:專用研磨管(上海凈信),研磨珠(上海凈信)
試劑:PBS緩沖液
實(shí)驗(yàn)步驟
1.將凈信專用研磨管和研磨球用乙醇清洗干凈,并滅菌滅酶;
2.將需要研磨的肌肉組織樣品剪成小塊備用。
3.放入準(zhǔn)備好的肌肉組織到研磨管中,加入研磨珠,蓋好蓋子;
4.將預(yù)冷好金屬適配器裝入凈信研磨儀,將研磨管裝入適配器。固定好。
4.設(shè)定好參數(shù),啟動(dòng)儀器至研磨程序結(jié)束。
實(shí)驗(yàn)前后對(duì)比

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